Expression of dentin sialoprotein in mouse, rat tooth |
| DSPP (gene); DSP (protein) |
| Species: mouse, rat |
| Tooth: molar and incisor |
| Method: in situ hybridization (radioactive), immunochemistry, reagent: 200nt / 1.2 kb / affinity purified anti-rat |
Late bell (differentiation) stage | |
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Expression: preameloblasts, preodontoblasts Transient expression in preameloblasts before the onset of secretion of enamel matrix.. Expression first visible in polarized odontoblasts.. |
Secretory stage | |
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Expression: odontoblasts, predentin, dentin No expression: outer enamel epithelium, stratum intermedia, stellate reticulum, ameloblasts, dental sac Discrepancy between immunostaining and in situ hybridization.. |
Root development | |
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Expression: odontoblasts, predentin, dentin No expression: cementoblasts, periodontal ligament |
| DSP is highly specific for the odontoblast lineage: not seen in bone, bone cells or cementum, cementum cells, and only very transient functional expression in preameloblasts. (Text by A.L.J.J. Bronckers). |
| DSP is expressed from the same transcript as dentin phosphoprotein. |
| Similar expression in rat tissues. |
| Source references: |
| Bronckers,A.L., D'Souza,R.N., Butler,W.T., Lyaruu,D.M., van Dijk,S., Gay,S., and, Wöltgens,J.H. (1993) Cell & Tissue Research 272(2):237-247 |
| Bronckers,A.L., Gay,S., Finkelman,R.D., and, Butler,W.T. (1987) Bone & Mineral 2(5):361-373 |
| Ritchie,H.H., Berry,J.E., Somerman,M.J., Hanks,C.T., Bronckers,A.L.J.J., Hotton, D, Papagerakis,P., Berdal,A., and, Butler,W.T. (1997) European Journal of Oral Sciences 105(5 Part 1):405-413 |
| Other references: |
| MacDougall,M., Simmons,D., Luan,X., Nydegger,J., Feng,J., and, Gu,T.T. (1997) Journal of Biological Chemistry 272(2):835-842 |