Expression of dentin sialoprotein in mouse, rat tooth

DSPP (gene); DSP (protein)

Species: mouse, rat
Tooth: molar and incisor
Method: in situ hybridization (radioactive), immunochemistry, reagent: 200nt / 1.2 kb / affinity purified anti-rat


Late bell
(differentiation) stage
Expression: preameloblasts, preodontoblasts

Transient expression in preameloblasts before the onset of secretion of enamel matrix..
Expression first visible in polarized odontoblasts..

Secretory stage
Expression: odontoblasts, predentin, dentin
No expression: outer enamel epithelium, stratum intermedia, stellate reticulum, ameloblasts, dental sac

Discrepancy between immunostaining and in situ hybridization..

Root development
Expression: odontoblasts, predentin, dentin
No expression: cementoblasts, periodontal ligament

DSP is highly specific for the odontoblast lineage: not seen in bone, bone cells or cementum, cementum cells, and only very transient functional expression in preameloblasts. (Text by A.L.J.J. Bronckers).
DSP is expressed from the same transcript as dentin phosphoprotein.
Similar expression in rat tissues.

Source references:
Bronckers,A.L., D'Souza,R.N., Butler,W.T., Lyaruu,D.M., van Dijk,S., Gay,S., and, Wöltgens,J.H. (1993) Cell & Tissue Research 272(2):237-247
Bronckers,A.L., Gay,S., Finkelman,R.D., and, Butler,W.T. (1987) Bone & Mineral 2(5):361-373
Ritchie,H.H., Berry,J.E., Somerman,M.J., Hanks,C.T., Bronckers,A.L.J.J., Hotton, D, Papagerakis,P., Berdal,A., and, Butler,W.T. (1997) European Journal of Oral Sciences 105(5 Part 1):405-413

Other references:
MacDougall,M., Simmons,D., Luan,X., Nydegger,J., Feng,J., and, Gu,T.T. (1997) Journal of Biological Chemistry 272(2):835-842


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Data last edited 03.03.1999 by M.P., page last created 10.12.2004 by P.N.