Expression of Runx2 in mouse tooth

Runt related transcription factor 2; Cbfa1; Core binding factor 1; Osf2; Osteoblast specific factor 2; Aml3; Pebp2alphaA

Species: mouse
Location in mouse genome: chromosome 17, 44002538 - 44208832 (UCSC, assembly October 2003)
Tooth: molar
Method: in situ hybridization (radioactive), probe: 336/600 b riboprobe


Initiation stage
Expression: dental mesenchyme
No expression: oral epithelium, dental epithelium

Bud stage
Expression: dental mesenchyme
No expression: oral epithelium, dental epithelium

Cap stage
Expression: dental papilla, dental sac
No expression: oral epithelium, outer enamel epithelium, inner enamel epithelium, enamel knot, stellate reticulum

Inducted in mesenchyme by epithelium, FGF4 or FGF8.
Shh expression was absent in the enamel knots of lower molars and reduced in the enamel knots of the upper molars in Runx2 null mutant mice (Åberg et al 2004). See expression of Sonic hedgehog.

Bell stage
Expression: dental papilla, dental sac
No expression: oral epithelium, outer enamel epithelium, inner enamel epithelium, stratum intermedia, stellate reticulum

Downregulated in dental papilla.

Late bell
(differentiation) stage
Expression: dental sac
No expression: outer enamel epithelium, stratum intermedia, stellate reticulum, preameloblasts, dental papilla, preodontoblasts

Secretory stage
Expression: ameloblasts, dental sac
No expression: outer enamel epithelium, stratum intermedia, stellate reticulum, dental papilla, odontoblasts

Transient expression in maturation stage ameloblasts.

Root development
Expression: periodontal ligament
No expression: dental papilla, odontoblasts, cementoblasts

Fgf3 expression was absent in the dental mesenchyme in Runx2 null mutant mice. (Åberg et al 2004) See expression of Fgf3.
Belongs to the Runt family of transcription factors.
Runx2 expression was absent in the mesenchyme in Msx1 null mutant mice. (Åberg et al 2004) See expression of Msx1.
In Runx2 null mutant mouse, tooth development is delayed and there is no differentiation.
Dominant mutations in human RUNX2 cause cleidocranial dysplasia and supernumerary teeth.

Source references:
D'Souza,R.N., Åberg,T., Gaikwad,J., Cavender,A., Owen,M., Karsenty,G., and, Thesleff,I. (1999) Development 126(13):2911-2920
Jiang,H., Sodek,J., Karsenty,G., Thomas,H., Ranly,D., and, Chen,J. (1999) Mechanisms of Development 81(1-2):169-173

Other references:
Komori,T., Yagi,H., Nomura,S., Yamaguchi,A., Sasaki,K., Deguchi,K., Shimizu,Y., Bronson,R.T., Gao,Y.H., Inada,M., Sato,M., Okamoto,R., Kitamura, Y, Yoshiki,S., and, Kishimoto,T. (1997) Cell 89(5):755-764
Lee,B., Thirunavukkarasu,K., Zhou,L., Pastore,L., Baldini,A., Hecht,J., Geoffroy,V., Ducy,P., and, Karsenty,G. (1997) Nature Genetics 16(3):307-310
Mundlos,S., Otto,F., Mundlos,C., Mulliken,J.B., Aylsworth,A.S., Albright,S., Lindhout,D., Cole,W.G., Henn,W., Knoll,J.H., Owen,M.J., Mertelsmann,R., Zabel, BU, and, Olsen,B.R. (1997) Cell 89(5):773-779
Otto,F., Thornell,A.P., Crompton,T., Denzel,A., Gilmour,K.C., Rosewell,I.R., Stamp,G.W., Beddington,R.S., Mundlos,S., Olsen,B.R., Selby,P.B., and, Owen,M.J. (1997) Cell 89(5):765-771
Zhou,G., Chen,Y., Zhou,L., Thirunavukkarasu,K., Hecht,J., Chitayat,D., Gelb,B.D., Pirinen,S., Berry,S.A., Greenberg,C.R., Karsenty,G., and, Lee,B. (1999) Human Molecular Genetics 8(12):2311-2316
Yamashiro,T., Åberg,T., Levanon,D., Groner,Y., and, Thesleff,I. (2002) Mech.Dev. 119 Suppl 1:S107-S110


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Data last edited 25.10.2003 by P.N., page last created 10.12.2004 by P.N.